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paav gfaabc1d gfp construct  (Addgene inc)


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    Addgene inc paav gfaabc1d gfp construct
    a An experimental scheme. b Latency to fall during rotarod training of control and Megf10 cKO (black: Megf10 fl/fl , n = 9 mice, red: Aldh1l1-CreERT2; Megf10 fl/fl , n = 13 mice; two-way ANOVA). c Representative confocal z-stack images showing astrocytic phagocytosis of corticostriatal presynapses in the DLS from control ( Megf10 fl/fl) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training (top: mCherry (red), eGFP (cyan), and S100B (blue); bottom: mCherry-only (magenta), S100B (cyan)). Scale bar, 10 μm. d Quantification of corticostriatal presynapses by astrocytes in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training ( Megf10 fl/fl ; 0 days: n = 6 mice, 5 days: n = 9 mice; Aldh1l1-CreERT2; Megf10 fl/fl ; 0 days: n = 6 mice, 5 days: n = 7 mice; one-way ANOVA). e Representative confocal z-stack images showing astrocytic phagocytosis of striatal postynapses in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training (top: mCherry (red), eGFP (cyan), and S100B (blue); bottom: mCherry-only (magenta), S100B (cyan)). Scale bar, 10 μm. f Quantification of striatal postsynaptic phagocytosis by astrocytes in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training ( Megf10 fl/fl ; 0 days: n = 5 mice, 5 days: n = 4 mice; Aldh1l1-CreERT2; Megf10 fl/fl ; 0 days: n = 8 mice, 5 days: n = 6 mice; one-way ANOVA). g Schematic illustration of the experiment to knock out Megf10 astrocytes conditionally in the striatum. h Latency to fall during the accelerating rotarod task of the control group <t>(</t> <t>GfaABC1D-Gfp</t> , green) and the Megf10 cKO group ( GfaABC1D-Cre , yellow) ( GfaABC1D-Gfp : n = 9 mice, GfaABC1D-Cre : n = 10 mice; two-way ANOVA). i A schematic illustration of the stimulation site in the corticostriatal pathway and the whole-cell patch-clamp recording in the DLS of a horizontal brain slice. Created in BioRender. Chung, W. (2026) https://BioRender.com/spxps4n . j Representative recording traces (left) and summary statistics (right) depicting the I/O relationships at corticostriatal excitatory synapses (Control, n = 19 cells from 5 mice; Megf10 cKO, n = 21 cells from 5 mice; RM two-way ANOVA). k Representative recording traces (left) and summary statistics (right) illustrating the AMPA/NMDA ratio at corticostriatal synapses (Control, n = 11 cells from 3 mice; Megf10 cKO, n = 11 cells from 4 mice; unpaired two-sided t -test). Data were presented as mean values ± SEM. AU arbitrary units.
    Paav Gfaabc1d Gfp Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paav+gfaabc1d+gfp+construct/AAV-CAG-GFP+(Plasmid+%2328014)/pmc12929717-242-3-12
    Average 93 stars, based on 51 article reviews
    paav gfaabc1d gfp construct - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Motor learning and dopamine-dependent striatal synaptic plasticity are controlled by astrocytic MEGF10"

    Article Title: Motor learning and dopamine-dependent striatal synaptic plasticity are controlled by astrocytic MEGF10

    Journal: Nature Communications

    doi: 10.1038/s41467-026-69129-1

    a An experimental scheme. b Latency to fall during rotarod training of control and Megf10 cKO (black: Megf10 fl/fl , n = 9 mice, red: Aldh1l1-CreERT2; Megf10 fl/fl , n = 13 mice; two-way ANOVA). c Representative confocal z-stack images showing astrocytic phagocytosis of corticostriatal presynapses in the DLS from control ( Megf10 fl/fl) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training (top: mCherry (red), eGFP (cyan), and S100B (blue); bottom: mCherry-only (magenta), S100B (cyan)). Scale bar, 10 μm. d Quantification of corticostriatal presynapses by astrocytes in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training ( Megf10 fl/fl ; 0 days: n = 6 mice, 5 days: n = 9 mice; Aldh1l1-CreERT2; Megf10 fl/fl ; 0 days: n = 6 mice, 5 days: n = 7 mice; one-way ANOVA). e Representative confocal z-stack images showing astrocytic phagocytosis of striatal postynapses in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training (top: mCherry (red), eGFP (cyan), and S100B (blue); bottom: mCherry-only (magenta), S100B (cyan)). Scale bar, 10 μm. f Quantification of striatal postsynaptic phagocytosis by astrocytes in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training ( Megf10 fl/fl ; 0 days: n = 5 mice, 5 days: n = 4 mice; Aldh1l1-CreERT2; Megf10 fl/fl ; 0 days: n = 8 mice, 5 days: n = 6 mice; one-way ANOVA). g Schematic illustration of the experiment to knock out Megf10 astrocytes conditionally in the striatum. h Latency to fall during the accelerating rotarod task of the control group ( GfaABC1D-Gfp , green) and the Megf10 cKO group ( GfaABC1D-Cre , yellow) ( GfaABC1D-Gfp : n = 9 mice, GfaABC1D-Cre : n = 10 mice; two-way ANOVA). i A schematic illustration of the stimulation site in the corticostriatal pathway and the whole-cell patch-clamp recording in the DLS of a horizontal brain slice. Created in BioRender. Chung, W. (2026) https://BioRender.com/spxps4n . j Representative recording traces (left) and summary statistics (right) depicting the I/O relationships at corticostriatal excitatory synapses (Control, n = 19 cells from 5 mice; Megf10 cKO, n = 21 cells from 5 mice; RM two-way ANOVA). k Representative recording traces (left) and summary statistics (right) illustrating the AMPA/NMDA ratio at corticostriatal synapses (Control, n = 11 cells from 3 mice; Megf10 cKO, n = 11 cells from 4 mice; unpaired two-sided t -test). Data were presented as mean values ± SEM. AU arbitrary units.
    Figure Legend Snippet: a An experimental scheme. b Latency to fall during rotarod training of control and Megf10 cKO (black: Megf10 fl/fl , n = 9 mice, red: Aldh1l1-CreERT2; Megf10 fl/fl , n = 13 mice; two-way ANOVA). c Representative confocal z-stack images showing astrocytic phagocytosis of corticostriatal presynapses in the DLS from control ( Megf10 fl/fl) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training (top: mCherry (red), eGFP (cyan), and S100B (blue); bottom: mCherry-only (magenta), S100B (cyan)). Scale bar, 10 μm. d Quantification of corticostriatal presynapses by astrocytes in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training ( Megf10 fl/fl ; 0 days: n = 6 mice, 5 days: n = 9 mice; Aldh1l1-CreERT2; Megf10 fl/fl ; 0 days: n = 6 mice, 5 days: n = 7 mice; one-way ANOVA). e Representative confocal z-stack images showing astrocytic phagocytosis of striatal postynapses in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training (top: mCherry (red), eGFP (cyan), and S100B (blue); bottom: mCherry-only (magenta), S100B (cyan)). Scale bar, 10 μm. f Quantification of striatal postsynaptic phagocytosis by astrocytes in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training ( Megf10 fl/fl ; 0 days: n = 5 mice, 5 days: n = 4 mice; Aldh1l1-CreERT2; Megf10 fl/fl ; 0 days: n = 8 mice, 5 days: n = 6 mice; one-way ANOVA). g Schematic illustration of the experiment to knock out Megf10 astrocytes conditionally in the striatum. h Latency to fall during the accelerating rotarod task of the control group ( GfaABC1D-Gfp , green) and the Megf10 cKO group ( GfaABC1D-Cre , yellow) ( GfaABC1D-Gfp : n = 9 mice, GfaABC1D-Cre : n = 10 mice; two-way ANOVA). i A schematic illustration of the stimulation site in the corticostriatal pathway and the whole-cell patch-clamp recording in the DLS of a horizontal brain slice. Created in BioRender. Chung, W. (2026) https://BioRender.com/spxps4n . j Representative recording traces (left) and summary statistics (right) depicting the I/O relationships at corticostriatal excitatory synapses (Control, n = 19 cells from 5 mice; Megf10 cKO, n = 21 cells from 5 mice; RM two-way ANOVA). k Representative recording traces (left) and summary statistics (right) illustrating the AMPA/NMDA ratio at corticostriatal synapses (Control, n = 11 cells from 3 mice; Megf10 cKO, n = 11 cells from 4 mice; unpaired two-sided t -test). Data were presented as mean values ± SEM. AU arbitrary units.

    Techniques Used: Control, Knock-Out, Patch Clamp, Slice Preparation

    Related Articles

    Construct:

    Article Title: Motor learning and dopamine-dependent striatal synaptic plasticity are controlled by astrocytic MEGF10
    Article Snippet: To generate pAAV- mDlx-DIO-PSD95.FingR-mCherry-eGFP-CCR5TC construct, PSD95.FingR-mCherry-eGFP construct from pAAV- mDlx-PSD95.FingR-mCherry-eGFP-CCR5TC and DIO construct from pAAV- EF1a-DIO-EYFP (Addgene #27056) were amplified by PCR, and both were subcloned into pAAV- mDlx-GFP-Fishell-1 . .. To generate the pAAV- GfaABC1D-GFP construct, the eGFP construct from pAAV- CAG-GFP (Addgene #28014) were amplified by PCR and subcloned into pAAV- GfaABC1D-Cre (Addgene #1056063). .. To generate pAAV- rTH-HA-hM3Dq and pAAV- rTH-HA-hM4Di constructs, HA-hM3Dq and HA-hM4Di construct from pAAV- GFAP-HA-hM3D(Gq)-IRES-mCitrine (Addgene #50470) and pAAV- GFAP-HA-hM4D(Gi)-IRES-mCitrine (Addgene #50471), respectively, were amplified by PCR and subcloned into pAAV- rTH-PI-Cre-SV40 (Addgene #107788).

    Amplification:

    Article Title: Motor learning and dopamine-dependent striatal synaptic plasticity are controlled by astrocytic MEGF10
    Article Snippet: To generate pAAV- mDlx-DIO-PSD95.FingR-mCherry-eGFP-CCR5TC construct, PSD95.FingR-mCherry-eGFP construct from pAAV- mDlx-PSD95.FingR-mCherry-eGFP-CCR5TC and DIO construct from pAAV- EF1a-DIO-EYFP (Addgene #27056) were amplified by PCR, and both were subcloned into pAAV- mDlx-GFP-Fishell-1 . .. To generate the pAAV- GfaABC1D-GFP construct, the eGFP construct from pAAV- CAG-GFP (Addgene #28014) were amplified by PCR and subcloned into pAAV- GfaABC1D-Cre (Addgene #1056063). .. To generate pAAV- rTH-HA-hM3Dq and pAAV- rTH-HA-hM4Di constructs, HA-hM3Dq and HA-hM4Di construct from pAAV- GFAP-HA-hM3D(Gq)-IRES-mCitrine (Addgene #50470) and pAAV- GFAP-HA-hM4D(Gi)-IRES-mCitrine (Addgene #50471), respectively, were amplified by PCR and subcloned into pAAV- rTH-PI-Cre-SV40 (Addgene #107788).

    Polymerase Chain Reaction:

    Article Title: Motor learning and dopamine-dependent striatal synaptic plasticity are controlled by astrocytic MEGF10
    Article Snippet: To generate pAAV- mDlx-DIO-PSD95.FingR-mCherry-eGFP-CCR5TC construct, PSD95.FingR-mCherry-eGFP construct from pAAV- mDlx-PSD95.FingR-mCherry-eGFP-CCR5TC and DIO construct from pAAV- EF1a-DIO-EYFP (Addgene #27056) were amplified by PCR, and both were subcloned into pAAV- mDlx-GFP-Fishell-1 . .. To generate the pAAV- GfaABC1D-GFP construct, the eGFP construct from pAAV- CAG-GFP (Addgene #28014) were amplified by PCR and subcloned into pAAV- GfaABC1D-Cre (Addgene #1056063). .. To generate pAAV- rTH-HA-hM3Dq and pAAV- rTH-HA-hM4Di constructs, HA-hM3Dq and HA-hM4Di construct from pAAV- GFAP-HA-hM3D(Gq)-IRES-mCitrine (Addgene #50470) and pAAV- GFAP-HA-hM4D(Gi)-IRES-mCitrine (Addgene #50471), respectively, were amplified by PCR and subcloned into pAAV- rTH-PI-Cre-SV40 (Addgene #107788).



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    paav gfaabc1d gfp construct  (Addgene inc)
    93
    Addgene inc paav gfaabc1d gfp construct
    a An experimental scheme. b Latency to fall during rotarod training of control and Megf10 cKO (black: Megf10 fl/fl , n = 9 mice, red: Aldh1l1-CreERT2; Megf10 fl/fl , n = 13 mice; two-way ANOVA). c Representative confocal z-stack images showing astrocytic phagocytosis of corticostriatal presynapses in the DLS from control ( Megf10 fl/fl) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training (top: mCherry (red), eGFP (cyan), and S100B (blue); bottom: mCherry-only (magenta), S100B (cyan)). Scale bar, 10 μm. d Quantification of corticostriatal presynapses by astrocytes in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training ( Megf10 fl/fl ; 0 days: n = 6 mice, 5 days: n = 9 mice; Aldh1l1-CreERT2; Megf10 fl/fl ; 0 days: n = 6 mice, 5 days: n = 7 mice; one-way ANOVA). e Representative confocal z-stack images showing astrocytic phagocytosis of striatal postynapses in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training (top: mCherry (red), eGFP (cyan), and S100B (blue); bottom: mCherry-only (magenta), S100B (cyan)). Scale bar, 10 μm. f Quantification of striatal postsynaptic phagocytosis by astrocytes in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training ( Megf10 fl/fl ; 0 days: n = 5 mice, 5 days: n = 4 mice; Aldh1l1-CreERT2; Megf10 fl/fl ; 0 days: n = 8 mice, 5 days: n = 6 mice; one-way ANOVA). g Schematic illustration of the experiment to knock out Megf10 astrocytes conditionally in the striatum. h Latency to fall during the accelerating rotarod task of the control group <t>(</t> <t>GfaABC1D-Gfp</t> , green) and the Megf10 cKO group ( GfaABC1D-Cre , yellow) ( GfaABC1D-Gfp : n = 9 mice, GfaABC1D-Cre : n = 10 mice; two-way ANOVA). i A schematic illustration of the stimulation site in the corticostriatal pathway and the whole-cell patch-clamp recording in the DLS of a horizontal brain slice. Created in BioRender. Chung, W. (2026) https://BioRender.com/spxps4n . j Representative recording traces (left) and summary statistics (right) depicting the I/O relationships at corticostriatal excitatory synapses (Control, n = 19 cells from 5 mice; Megf10 cKO, n = 21 cells from 5 mice; RM two-way ANOVA). k Representative recording traces (left) and summary statistics (right) illustrating the AMPA/NMDA ratio at corticostriatal synapses (Control, n = 11 cells from 3 mice; Megf10 cKO, n = 11 cells from 4 mice; unpaired two-sided t -test). Data were presented as mean values ± SEM. AU arbitrary units.
    Paav Gfaabc1d Gfp Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paav+gfaabc1d+gfp+construct/AAV-CAG-GFP+(Plasmid+%2328014)/pmc12929717-242-3-12
    Average 93 stars, based on 1 article reviews
    paav gfaabc1d gfp construct - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    a An experimental scheme. b Latency to fall during rotarod training of control and Megf10 cKO (black: Megf10 fl/fl , n = 9 mice, red: Aldh1l1-CreERT2; Megf10 fl/fl , n = 13 mice; two-way ANOVA). c Representative confocal z-stack images showing astrocytic phagocytosis of corticostriatal presynapses in the DLS from control ( Megf10 fl/fl) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training (top: mCherry (red), eGFP (cyan), and S100B (blue); bottom: mCherry-only (magenta), S100B (cyan)). Scale bar, 10 μm. d Quantification of corticostriatal presynapses by astrocytes in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training ( Megf10 fl/fl ; 0 days: n = 6 mice, 5 days: n = 9 mice; Aldh1l1-CreERT2; Megf10 fl/fl ; 0 days: n = 6 mice, 5 days: n = 7 mice; one-way ANOVA). e Representative confocal z-stack images showing astrocytic phagocytosis of striatal postynapses in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training (top: mCherry (red), eGFP (cyan), and S100B (blue); bottom: mCherry-only (magenta), S100B (cyan)). Scale bar, 10 μm. f Quantification of striatal postsynaptic phagocytosis by astrocytes in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training ( Megf10 fl/fl ; 0 days: n = 5 mice, 5 days: n = 4 mice; Aldh1l1-CreERT2; Megf10 fl/fl ; 0 days: n = 8 mice, 5 days: n = 6 mice; one-way ANOVA). g Schematic illustration of the experiment to knock out Megf10 astrocytes conditionally in the striatum. h Latency to fall during the accelerating rotarod task of the control group ( GfaABC1D-Gfp , green) and the Megf10 cKO group ( GfaABC1D-Cre , yellow) ( GfaABC1D-Gfp : n = 9 mice, GfaABC1D-Cre : n = 10 mice; two-way ANOVA). i A schematic illustration of the stimulation site in the corticostriatal pathway and the whole-cell patch-clamp recording in the DLS of a horizontal brain slice. Created in BioRender. Chung, W. (2026) https://BioRender.com/spxps4n . j Representative recording traces (left) and summary statistics (right) depicting the I/O relationships at corticostriatal excitatory synapses (Control, n = 19 cells from 5 mice; Megf10 cKO, n = 21 cells from 5 mice; RM two-way ANOVA). k Representative recording traces (left) and summary statistics (right) illustrating the AMPA/NMDA ratio at corticostriatal synapses (Control, n = 11 cells from 3 mice; Megf10 cKO, n = 11 cells from 4 mice; unpaired two-sided t -test). Data were presented as mean values ± SEM. AU arbitrary units.

    Journal: Nature Communications

    Article Title: Motor learning and dopamine-dependent striatal synaptic plasticity are controlled by astrocytic MEGF10

    doi: 10.1038/s41467-026-69129-1

    Figure Lengend Snippet: a An experimental scheme. b Latency to fall during rotarod training of control and Megf10 cKO (black: Megf10 fl/fl , n = 9 mice, red: Aldh1l1-CreERT2; Megf10 fl/fl , n = 13 mice; two-way ANOVA). c Representative confocal z-stack images showing astrocytic phagocytosis of corticostriatal presynapses in the DLS from control ( Megf10 fl/fl) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training (top: mCherry (red), eGFP (cyan), and S100B (blue); bottom: mCherry-only (magenta), S100B (cyan)). Scale bar, 10 μm. d Quantification of corticostriatal presynapses by astrocytes in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training ( Megf10 fl/fl ; 0 days: n = 6 mice, 5 days: n = 9 mice; Aldh1l1-CreERT2; Megf10 fl/fl ; 0 days: n = 6 mice, 5 days: n = 7 mice; one-way ANOVA). e Representative confocal z-stack images showing astrocytic phagocytosis of striatal postynapses in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training (top: mCherry (red), eGFP (cyan), and S100B (blue); bottom: mCherry-only (magenta), S100B (cyan)). Scale bar, 10 μm. f Quantification of striatal postsynaptic phagocytosis by astrocytes in the DLS from control ( Megf10 fl/fl ) and Megf10 cKO ( Aldh1l1-CreERT2; Megf10 fl/fl ) mice during rotarod training ( Megf10 fl/fl ; 0 days: n = 5 mice, 5 days: n = 4 mice; Aldh1l1-CreERT2; Megf10 fl/fl ; 0 days: n = 8 mice, 5 days: n = 6 mice; one-way ANOVA). g Schematic illustration of the experiment to knock out Megf10 astrocytes conditionally in the striatum. h Latency to fall during the accelerating rotarod task of the control group ( GfaABC1D-Gfp , green) and the Megf10 cKO group ( GfaABC1D-Cre , yellow) ( GfaABC1D-Gfp : n = 9 mice, GfaABC1D-Cre : n = 10 mice; two-way ANOVA). i A schematic illustration of the stimulation site in the corticostriatal pathway and the whole-cell patch-clamp recording in the DLS of a horizontal brain slice. Created in BioRender. Chung, W. (2026) https://BioRender.com/spxps4n . j Representative recording traces (left) and summary statistics (right) depicting the I/O relationships at corticostriatal excitatory synapses (Control, n = 19 cells from 5 mice; Megf10 cKO, n = 21 cells from 5 mice; RM two-way ANOVA). k Representative recording traces (left) and summary statistics (right) illustrating the AMPA/NMDA ratio at corticostriatal synapses (Control, n = 11 cells from 3 mice; Megf10 cKO, n = 11 cells from 4 mice; unpaired two-sided t -test). Data were presented as mean values ± SEM. AU arbitrary units.

    Article Snippet: To generate the pAAV- GfaABC1D-GFP construct, the eGFP construct from pAAV- CAG-GFP (Addgene #28014) were amplified by PCR and subcloned into pAAV- GfaABC1D-Cre (Addgene #1056063).

    Techniques: Control, Knock-Out, Patch Clamp, Slice Preparation